Hotspot

infB

Genome · 91571-93850 · 2280 bp

Back to Laurencia

Pi 0.113

MarkerSeek score 77.10

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2280
variable_sites: 448
pi: 0.113
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	ACGTTCAACAGGACTAAGCTC	CCCTTCAGATTGTGCTGACA	47.6	50.0	58.0	57.8	2697 bp	0.25	40.3
infB_p2	AGACGTTCAACAGGACTAAGC	CCCTTCAGATTGTGCTGACA	47.6	50.0	58.0	57.8	2699 bp	0.25	40.3
infB_p3	GACGTTCAACAGGACTAAGCT	CCCTTCAGATTGTGCTGACA	47.6	50.0	58.0	57.8	2698 bp	0.25	40.3
infB_p4	ACGTTCAACAGGACTAAGCTC	TTCCCCTTCAGATTGTGCTG	47.6	50.0	58.0	57.8	2700 bp	0.25	40.1
infB_p5	GACGTTCAACAGGACTAAGCT	TTCCCCTTCAGATTGTGCTG	47.6	50.0	58.0	57.8	2701 bp	0.25	40.1