Hotspot

dnaB

Genome · 150111-151937 · 1827 bp

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Pi 0.127

MarkerSeek score 78.60

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1827
variable_sites: 387
pi: 0.127
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	GCAGTCGTAGATGTTGCTGA	TACCAAACGGTCTTGCTAGC	50.0	50.0	58.0	57.9	2516 bp	0.25	49.3
dnaB_p2	TCAGTCCACTGCTCTACCAA	TACCAAACGGTCTTGCTAGC	50.0	50.0	58.0	57.9	2321 bp	0.25	49.2
dnaB_p3	TCCACTGCTCTACCAACTGA	TACCAAACGGTCTTGCTAGC	50.0	50.0	58.0	57.9	2317 bp	0.25	49.2
dnaB_p4	TTCAGTCCACTGCTCTACCA	TACCAAACGGTCTTGCTAGC	50.0	50.0	58.0	57.9	2322 bp	0.25	49.2
dnaB_p5	AGTCCACTGCTCTACCAACT	TACCAAACGGTCTTGCTAGC	50.0	50.0	58.0	57.9	2319 bp	0.25	49.2