Hotspot

carA

Genome · 13577-14746 · 1170 bp

Back to Laurencia

Pi 0.0901

MarkerSeek score 79.70

Primer available yes

Label carA

Feature Metadata

label_name: carA
feature_type: gene
region: Genome
length_bp: 1170
variable_sites: 183
pi: 0.0901
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA_p1	TCTCCAGATAAGTGGGGTGG	GCACCAGAAGGACACTTACC	55.0	55.0	58.1	58.2	1893-1905 bp	0.75	76.1
carA_p2	AAGCGGGAGAACAGGTTTTT	TGGACAACAAAAGCACCAGA	45.0	45.0	57.9	57.9	1659 bp	0.25	48.4
carA_p3	TCTCCAGATAAGTGGGGTGG	TGGACAACAAAAGCACCAGA	55.0	45.0	58.1	57.9	1917 bp	0.25	47.9
carA_p4	AAGCGGGAGAACAGGTTTTT	GCACCAGAAGGACACTTACC	45.0	55.0	57.9	58.2	1647 bp	0.25	47.9
carA_p5	TGGCGCTAAGTTGGATGAAA	TGGACAACAAAAGCACCAGA	45.0	45.0	57.8	57.9	1487 bp	0.25	47.4