Hotspot

psbI-ycf39

Genome · 70020-70777 · 758 bp

Back to Laurencia

Pi 0.247
MarkerSeek score 80.10
Primer available yes
Label psbI-ycf39

Feature Metadata

label_name
psbI-ycf39
feature_type
igs
region
Genome
length_bp
758
variable_sites
284
pi
0.247
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.87
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-ycf39_p1 TGGATTACGTGAAGGATCGT ACCCCAGTATGCTTTACCTG 45.0 50.0 56.4 56.9 1365 bp 0.25 28.1
psbI-ycf39_p2 TGGATTACGTGAAGGATCGT CCCCAGTATGCTTTACCTGT 45.0 50.0 56.4 56.9 1364 bp 0.25 28.1
psbI-ycf39_p3 TGGATTACGTGAAGGATCGTT ACCCCAGTATGCTTTACCTG 42.9 50.0 57.1 56.9 1365 bp 0.25 26.1
psbI-ycf39_p4 TGGATTACGTGAAGGATCGTT CCCCAGTATGCTTTACCTGT 42.9 50.0 57.1 56.9 1364 bp 0.25 26.1
psbI-ycf39_p5 TTGGATTACGTGAAGGATCGT CCCCAGTATGCTTTACCTGT 42.9 50.0 57.1 56.9 1365 bp 0.25 26.1