Hotspot

mat-cemA

Genome · 67519-68348 · 830 bp

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Pi 0.0955

MarkerSeek score 81.60

Primer available yes

Label mat-cemA

Feature Metadata

label_name: mat-cemA
feature_type: igs
region: Genome
length_bp: 830
variable_sites: 139
pi: 0.0955
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
mat-cemA_p1	GCTTAGCTTTGCAAACAGGT	CCTTCTGCTGTTGCGACTTA	45.0	50.0	57.2	58.2	1179 bp	0.25	42.0
mat-cemA_p2	GCTTAGCTTTGCAAACAGGT	GGCGCAACAAAGGATTTCTG	45.0	50.0	57.2	58.3	1582 bp	0.25	41.2
mat-cemA_p3	GCTTAGCTTTGCAAACAGGT	TATCTCCTTCTGCTGTTGCG	45.0	50.0	57.2	57.7	1184 bp	0.25	41.2
mat-cemA_p4	GCTTAGCTTTGCAAACAGGT	ATGGCGCAACAAAGGATTTC	45.0	45.0	57.2	57.6	1584 bp	0.25	40.6
mat-cemA_p5	GCTTAGCTTTGCAAACAGGT	AGATATGGCGCAACAAAGGA	45.0	45.0	57.2	57.6	1588 bp	0.25	40.2