Hotspot

trnL(tag)-trnN(gtt)

Genome · 23407-23545 · 139 bp

Back to Laurencia

Pi 0.165

MarkerSeek score 81.10

Primer available yes

Label trnL(tag)-trnN(gtt)

Feature Metadata

label_name: trnL(tag)-trnN(gtt)
feature_type: igs
region: Genome
length_bp: 139
variable_sites: 35
pi: 0.165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL(tag)-trnN(gtt)_p1	TCTAGCGTGTCTGCCAATTC	ACTCCCCAGGAAGGATTTGA	50.0	50.0	58.0	57.9	230-245 bp	1.0	91.4
trnL(tag)-trnN(gtt)_p2	TCTAGCGTGTCTGCCAATTC	CCCCAGGAAGGATTTGAACC	50.0	55.0	58.0	58.1	227-242 bp	1.0	90.7
trnL(tag)-trnN(gtt)_p3	TCTGCCAATTCCACCACATC	ACTCCCCAGGAAGGATTTGA	50.0	50.0	58.2	57.9	221-236 bp	1.0	90.2
trnL(tag)-trnN(gtt)_p4	TCTGCCAATTCCACCACATC	CCCCAGGAAGGATTTGAACC	50.0	55.0	58.2	58.1	218-233 bp	1.0	89.5
trnL(tag)-trnN(gtt)_p5	TCTAGCGTGTCTGCCAATTC	ACCACTGAGCTACTGAGGAA	50.0	50.0	58.0	57.7	176-191 bp	1.0	89.4