Hotspot

trnA(tgc)-rnl

Genome · 1691-1772 · 82 bp

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Pi 0.0802

MarkerSeek score 80.10

Primer available yes

Label trnA(tgc)-rnl

Feature Metadata

label_name: trnA(tgc)-rnl
feature_type: igs
region: Genome
length_bp: 82
variable_sites: 11
pi: 0.0802
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnA(tgc)-rnl_p1	GGTTCGAGTCCGCTTATCTC	ATGTTTCGTCGGTAGTCACG	55.0	50.0	57.9	58.0	173-175 bp	1.0	84.8
trnA(tgc)-rnl_p2	GTTCGAGTCCGCTTATCTCC	ATGTTTCGTCGGTAGTCACG	55.0	50.0	57.9	58.0	172-174 bp	1.0	84.8
trnA(tgc)-rnl_p3	TTCGAGTCCGCTTATCTCCA	ATGTTTCGTCGGTAGTCACG	50.0	50.0	58.2	58.0	171-173 bp	1.0	83.9
trnA(tgc)-rnl_p4	GTTCGAGTCCGCTTATCTCC	CGCCCTTCATCGCTTCTAAA	55.0	50.0	57.9	58.3	154-156 bp	1.0	82.9
trnA(tgc)-rnl_p5	GCTTTTGCAAGGCAGATGTC	ATGTTTCGTCGGTAGTCACG	50.0	50.0	58.3	58.0	196-198 bp	1.0	82.7