Hotspot

ycf1

SSC · 109866-114863 · 4998 bp

Back to Launaea

Pi 0.0204
MarkerSeek score 52.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
4998
variable_sites
102
pi
0.0204
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 GGTCACTTTCTATTGATGAAATGGA GGGTGACGGTAGTCTACCTA 36.0 55.0 57.4 57.3 192 bp 1.0 45.4
ycf1_p2 GGTCACTTTCTATTGATGAAATGGA GGGTGACGGTAGTCTACCTAA 36.0 52.4 57.4 58.0 192 bp 1.0 45.4
ycf1_p3 GGTCACTTTCTATTGATGAAATGG GGGTGACGGTAGTCTACCTA 37.5 55.0 56.1 57.3 192 bp 1.0 45.4
ycf1_p4 GGTCACTTTCTATTGATGAAATGGA TGGGTGACGGTAGTCTACCT 36.0 55.0 57.4 59.3 193 bp 1.0 45.4
ycf1_p5 GGTCACTTTCTATTGATGAAATGG GGGTGACGGTAGTCTACCTAA 37.5 52.4 56.1 58.0 192 bp 1.0 45.4