Hotspot

ndhG

SSC · 119894-120424 · 531 bp

Back to Launaea

Pi 0.0188

MarkerSeek score 58.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 10
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TTTTTCCTGACCGGGTCAAT	AGGAAATGCATGTGATAGCTCA	45.0	40.9	57.3	57.8	650 bp	1.0	58.6
ndhG_p2	CCTGACCGGGTCAATAAAGT	AGGAAATGCATGTGATAGCTCA	50.0	40.9	57.2	57.8	645 bp	1.0	57.7
ndhG_p3	CCTGACCGGGTCAATAAAGTT	AGGAAATGCATGTGATAGCTCA	47.6	40.9	57.9	57.8	645 bp	1.0	55.4
ndhG_p4	TCCTGACCGGGTCAATAAAG	AGGAAATGCATGTGATAGCTCA	50.0	40.9	56.9	57.8	646 bp	1.0	55.4
ndhG_p5	TTTTTCCTGACCGGGTCAAT	GGAAATGCATGTGATAGCTCA	45.0	42.9	57.3	56.4	649 bp	1.0	54.9