Hotspot

rrn5-trnR

IRb · 108559-108815 · 257 bp

Back to Launaea

Pi 0.0195

MarkerSeek score 63.70

Primer available yes

Label rrn5-trnR

Feature Metadata

label_name: rrn5-trnR
feature_type: igs
region: IRb
length_bp: 257
variable_sites: 5
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-trnR_p1	GGTGACGATACTGTAGGGGA	CCACGTGCTCTAATCCTCTG	55.0	55.0	57.9	57.8	341-343 bp	1.0	78.9
rrn5-trnR_p2	AATCCATCCCGAACTTGGTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	376-378 bp	1.0	78.3
rrn5-trnR_p3	ACCAATCCATCCCGAACTTG	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	379-381 bp	1.0	78.3
rrn5-trnR_p4	CAATCCATCCCGAACTTGGT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	377-379 bp	1.0	78.3
rrn5-trnR_p5	CACCAATCCATCCCGAACTT	CCACGTGCTCTAATCCTCTG	50.0	55.0	57.9	57.8	380-382 bp	1.0	78.3