Hotspot

petB-petD

LSC · 77156-78001 · 846 bp

Back to Launaea

Pi 0.00946

MarkerSeek score 64.20

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 8
pi: 0.00946
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CCGCTTCTTACTGCCGTATT	TATAGATCCATCCCCCGGAG	50.0	55.0	58.1	57.2	952 bp	1.0	73.4
petB-petD_p2	CCGCTTCTTACTGCCGTATT	ACCTTTAGCCAATTTCGCTCT	50.0	42.9	58.1	57.9	1026 bp	1.0	71.5
petB-petD_p3	CCGCTTCTTACTGCCGTATT	CTTATAGATCCATCCCCCGGA	50.0	52.4	58.1	57.9	954 bp	1.0	71.2
petB-petD_p4	CCGCTTCTTACTGCCGTATT	TCTTATAGATCCATCCCCCGG	50.0	52.4	58.1	57.9	955 bp	1.0	71.2
petB-petD_p5	CCGCTTCTTACTGCCGTATT	TTATAGATCCATCCCCCGGAG	50.0	52.4	58.1	57.9	953 bp	1.0	71.2