Hotspot

accD-psaI

LSC · 59339-60162 · 824 bp

Back to Launaea

Pi 0.0205

MarkerSeek score 64.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 15
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	879-962 bp	1.0	65.6
accD-psaI_p2	AAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	878-961 bp	1.0	65.6
accD-psaI_p3	TCAACTCCACACTTTCTTTCCT	AAAGAAGCCATTGCGATTGC	40.9	45.0	57.5	58.0	855-938 bp	1.0	65.3
accD-psaI_p4	AAAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	38.1	45.0	56.3	58.0	879-962 bp	1.0	63.9
accD-psaI_p5	AAAAGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	40.0	45.0	55.5	58.3	868-951 bp	1.0	63.5