Hotspot

trnT-psbD

LSC · 31556-32932 · 1377 bp

Back to Launaea

Pi 0.012

MarkerSeek score 65.30

Primer available yes

Label trnT-psbD

Feature Metadata

label_name: trnT-psbD
feature_type: igs
region: LSC
length_bp: 1377
variable_sites: 15
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-psbD_p1	CGTAAGCCCTCGGTTCAAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1401-1512 bp	1.0	81.8
trnT-psbD_p2	TCCATGAACCAAGCCCTTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1457-1568 bp	1.0	80.7
trnT-psbD_p3	TTCCATGAACCAAGCCCTTT	GGACCAGCCTACAAAAACGA	45.0	50.0	57.6	58.1	1458-1569 bp	1.0	80.7
trnT-psbD_p4	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1391-1502 bp	1.0	80.7
trnT-psbD_p5	TAAGCCCTCGGTTCAAATCC	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	1399-1510 bp	1.0	80.6