Hotspot

atpI-atpH

LSC · 25381-26548 · 1168 bp

Back to Launaea

Pi 0.0115
MarkerSeek score 68.60
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1168
variable_sites
13
pi
0.0115
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 TCCCTGTCATGTTCCTTGGA ATAACGGAAGCGGCAGAAAT 50.0 45.0 58.3 58.0 1266-1304 bp 1.0 80.9
atpI-atpH_p2 TCCCTGTCATGTTCCTTGGA TACCTTGACCAACTCCAGGT 50.0 50.0 58.3 57.9 1322-1360 bp 1.0 80.2
atpI-atpH_p3 TCCCTGTCATGTTCCTTGGA AGCCAATCCAGCAGCAATAA 50.0 45.0 58.3 57.9 1282-1320 bp 1.0 80.0
atpI-atpH_p4 TCCCTGTCATGTTCCTTGGA AATAACGGAAGCGGCAGAAA 50.0 45.0 58.3 58.2 1267-1305 bp 1.0 79.8
atpI-atpH_p5 TCCCTGTCATGTTCCTTGGA GCAGTACCTTGACCAACTCC 50.0 55.0 58.3 58.2 1326-1364 bp 1.0 79.6