Hotspot

trnC-petN

LSC · 9726-10557 · 832 bp

Back to Launaea

Pi 0.0263

MarkerSeek score 62.80

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 21
pi: 0.0263
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	874-889 bp	1.0	83.3
trnC-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	892-907 bp	1.0	81.0
trnC-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	873-888 bp	1.0	77.4
trnC-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	882-897 bp	1.0	77.3
trnC-petN_p5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	931-946 bp	1.0	76.4