Hotspot

trnH-psbA

LSC · 78-485 · 408 bp

Back to Launaea

Pi 0.0637

MarkerSeek score 85.20

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 408
variable_sites: 26
pi: 0.0637
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	GATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	55.0	55.0	58.1	58.3	526-537 bp	1.0	80.2
trnH-psbA_p2	CAATCCACTGCCTTGATCCA	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	540-551 bp	1.0	80.1
trnH-psbA_p3	AATCCACTGCCTTGATCCAC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	539-550 bp	1.0	80.1
trnH-psbA_p4	ACAATCCACTGCCTTGATCC	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	541-552 bp	1.0	80.0
trnH-psbA_p5	GATCCACTTGGCTACATCCG	ACCGTGCTAACCTTGGTATG	55.0	50.0	58.1	57.6	527-538 bp	1.0	79.7