Pi
0.00144
MarkerSeek score
59.60
Primer available
yes
Label
cemA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| cemA_p1 | GAACCCATGGATCTATTTCCCA | GGACGAATACCTTAAAGAGCT | 45.5 | 42.9 | 57.5 | 55.1 | 913 bp | 1.0 | 40.1 |
| cemA_p2 | TGAACCCATGGATCTATTTCCC | GGACGAATACCTTAAAGAGCT | 45.5 | 42.9 | 57.5 | 55.1 | 914 bp | 1.0 | 40.1 |
| cemA_p3 | ACCCATGGATCTATTTCCCA | GGACGAATACCTTAAAGAGCT | 45.0 | 42.9 | 55.5 | 55.1 | 911 bp | 1.0 | 40.1 |
| cemA_p4 | TCTCGGATCTAGAAGGAAAGGA | GGACGAATACCTTAAAGAGCT | 45.5 | 42.9 | 57.5 | 55.1 | 871 bp | 1.0 | 40.1 |
| cemA_p5 | AACCCATGGATCTATTTCCCA | GGACGAATACCTTAAAGAGCT | 42.9 | 42.9 | 56.4 | 55.1 | 912 bp | 1.0 | 40.1 |