Hotspot

ccsA-ndhD

SSC · 119594-119809 · 216 bp

Back to Latania

Pi 0.0139

MarkerSeek score 60.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 216
variable_sites: 3
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAATTCGGCACTTATAGCT	GTTGACAGGGTACAAGCCAT	42.9	50.0	57.5	57.8	381 bp	1.0	67.4
ccsA-ndhD_p2	ACGAATTCGGCACTTATAGCT	ACAGGGTACAAGCCATCCTA	42.9	50.0	57.5	57.7	377 bp	1.0	66.9
ccsA-ndhD_p3	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCC	42.9	55.0	57.5	58.5	383 bp	1.0	65.2
ccsA-ndhD_p4	ACGAATTCGGCACTTATAGCT	TTGACAGGGTACAAGCCATC	42.9	50.0	57.5	57.5	380 bp	1.0	65.1
ccsA-ndhD_p5	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	42.9	50.0	57.5	57.5	384 bp	1.0	64.6