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petA-psbJ

LSC · 65943-66987 · 1045 bp

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Pi 0.00766

MarkerSeek score 63.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1045
variable_sites: 8
pi: 0.00766
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1197-1202 bp	1.0	77.7
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1193-1198 bp	1.0	76.8
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1180-1185 bp	1.0	76.8
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1196-1201 bp	1.0	76.8
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1194-1199 bp	1.0	76.6