Hotspot

rps16

LSC · 4700-5818 · 1119 bp

Back to Lasianthus

Pi 0.00542

MarkerSeek score 63.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1119
variable_sites: 9
pi: 0.00542
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATACACAAAGGACAAGGGGG	ACCGTTTCCAAATCAAGAGGA	50.0	42.9	57.1	57.5	1245-1259 bp	1.0	62.3
rps16_p2	GATACACAAAGGACAAGGGGG	ACCGTTTCCAAATCAAGAGGA	52.4	42.9	58.3	57.5	1246-1260 bp	1.0	59.2
rps16_p3	ATACACAAAGGACAAGGGGG	ACCGTTTCCAAATCAAGAGG	50.0	45.0	57.1	55.9	1245-1259 bp	1.0	57.8
rps16_p4	TTTACCCCCACCCTCTTTTT	ACCGTTTCCAAATCAAGAGGA	45.0	42.9	56.5	57.5	1168-1180 bp	1.0	57.6
rps16_p5	ATACACAAAGGACAAGGGGG	ACCGTTTCCAAATCAAGAGGAA	50.0	40.9	57.1	58.2	1245-1259 bp	1.0	56.9