Hotspot

ndhG-ndhI

SSC · 123865-124500 · 636 bp

Back to Lasianthus

Pi 0.0101

MarkerSeek score 53.80

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 636
variable_sites: 9
pi: 0.0101
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	GCGAAGTAACTGCATCTCCA	CCGGTACATTGGTCAAGGTT	50.0	50.0	58.0	57.8	1354-1409 bp	1.0	82.7
ndhG-ndhI_p2	TGCAGCAGCTACAAAGTAGG	CCGGTACATTGGTCAAGGTT	50.0	50.0	57.9	57.8	1233-1288 bp	1.0	82.3
ndhG-ndhI_p3	GCGAAGTAACTGCATCTCCA	CAACAAACAGTACGAGCTGC	50.0	50.0	58.0	57.7	1374-1429 bp	1.0	81.8
ndhG-ndhI_p4	CTGCATCTCCAACAGTCCAA	CCGGTACATTGGTCAAGGTT	50.0	50.0	57.8	57.8	1345-1400 bp	1.0	81.3
ndhG-ndhI_p5	AACTGCATCTCCAACAGTCC	CCGGTACATTGGTCAAGGTT	50.0	50.0	57.8	57.8	1347-1402 bp	1.0	81.3