Hotspot

rpl32-trnL-UAG

SSC · 118165-119340 · 1176 bp

Back to Lasianthus

Pi 0.00486

MarkerSeek score 66.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1176
variable_sites: 7
pi: 0.00486
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAGAGGGTATTGGACAGC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.5	57.9	1312-1361 bp	1.0	78.3
rpl32-trnL-UAG_p2	AGGAGAGGGTATTGGACAGC	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.5	57.8	1305-1354 bp	1.0	77.9
rpl32-trnL-UAG_p3	AGGAGAGGGTATTGGACAGC	CCTAAGAGCAGCGTGTCTAC	55.0	55.0	58.5	57.8	1311-1360 bp	1.0	77.8
rpl32-trnL-UAG_p4	AGGAGAGGGTATTGGACAGC	CTAAGAGCAGCGTGTCTACC	55.0	55.0	58.5	57.8	1310-1359 bp	1.0	77.8
rpl32-trnL-UAG_p5	TGGACAGCATTAAAGGCCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.4	57.9	1300-1349 bp	1.0	77.5