Hotspot

psaJ-rpl33

LSC · 68913-69454 · 542 bp

Back to Lasianthus

Pi 0.00997

MarkerSeek score 69.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 8
pi: 0.00997
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGATGCGTTAACATTCCCCT	ATCTGGAAATGCCCGTTGAT	50.0	45.0	57.9	57.6	639-674 bp	1.0	81.0
psaJ-rpl33_p2	GGGGCTTTAGCGGGTTTATT	ATCTGGAAATGCCCGTTGAT	50.0	45.0	58.2	57.6	683-718 bp	1.0	79.5
psaJ-rpl33_p3	GGATGCGTTAACATTCCCCT	TTTGGAACACAGCTGGTACA	50.0	45.0	57.9	57.3	604-639 bp	1.0	78.8
psaJ-rpl33_p4	TCCGGATGCGTTAACATTCC	ATCTGGAAATGCCCGTTGAT	50.0	45.0	58.3	57.6	642-677 bp	1.0	78.7
psaJ-rpl33_p5	GGGGCTTTAGCGGGTTTATT	TTTGGAACACAGCTGGTACA	50.0	45.0	58.2	57.3	648-683 bp	1.0	77.4