Hotspot

ycf4-cemA

LSC · 61697-62572 · 876 bp

Back to Lasianthus

Pi 0.0247

MarkerSeek score 75.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 32
pi: 0.0247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGAGCCATTCCCTTAACTCG	GAGAAAGACACCAGGGCAAA	55.0	50.0	58.0	58.0	897-1057 bp	1.0	86.0
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAAGACACCAGGGCAAA	45.0	50.0	58.1	58.0	828-988 bp	1.0	85.8
ycf4-cemA_p3	AAAAGGTGCTGAATTGGCCT	GAGAAAGACACCAGGGCAAA	45.0	50.0	58.3	58.0	841-1001 bp	1.0	84.3
ycf4-cemA_p4	GGAGCCATTCCCTTAACTCG	AAAGAGAAAGACACCAGGGC	55.0	50.0	58.0	57.7	900-1060 bp	1.0	84.0
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	AAAGAGAAAGACACCAGGGC	45.0	50.0	58.1	57.7	831-991 bp	1.0	83.7