Hotspot

psaI-ycf4

LSC · 60604-61141 · 538 bp

Back to Lasianthus

Pi 0.0305

MarkerSeek score 77.20

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 538
variable_sites: 24
pi: 0.0305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	AGGCCCAGCAGAAATTACTT	45.0	45.0	58.0	57.1	663-675 bp	1.0	77.0
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	AAGGCCCAGCAGAAATTACT	45.0	45.0	58.0	57.1	664-676 bp	1.0	76.9
psaI-ycf4_p3	CGGCAATTGCAATGGTTTCT	AAGGCCCAGCAGAAATTACT	45.0	45.0	57.9	57.1	659-671 bp	1.0	76.5
psaI-ycf4_p4	CGGCAATTGCAATGGTTTCT	AGGCCCAGCAGAAATTACTT	45.0	45.0	57.9	57.1	658-670 bp	1.0	76.5
psaI-ycf4_p5	TAGGCCTAGTATTTCCGGCA	AGGCCCAGCAGAAATTACTT	50.0	45.0	57.9	57.1	673-685 bp	1.0	76.4