Hotspot

psbC-trnS-UGA

LSC · 35994-36278 · 285 bp

Back to Lasianthus

Pi 0.0152

MarkerSeek score 60.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 285
variable_sites: 6
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGGTTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	422-446 bp	1.0	83.9
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GTCTTTCGGAGAGATGGCTG	50.0	55.0	58.1	58.1	430-454 bp	1.0	83.5
psbC-trnS-UGA_p3	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	463-487 bp	1.0	83.3
psbC-trnS-UGA_p4	GCAGCAGGGTTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.1	57.9	402-426 bp	1.0	83.1
psbC-trnS-UGA_p5	TCGTAGGTCATTTGTGGCAC	GTCTTTCGGAGAGATGGCTG	50.0	55.0	58.2	58.1	471-495 bp	1.0	82.9