Hotspot

atpI-rps2

LSC · 15117-15447 · 331 bp

Back to Lasianthus

Pi 0.0234

MarkerSeek score 72.30

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 9
pi: 0.0234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	CTTGTCCGTGGACTTGGAAA	GTGTTCGCAATTTGTGAGGG	50.0	50.0	58.0	57.9	473-492 bp	1.0	84.8
atpI-rps2_p2	CTTCCACACCGGATACTTCG	GTGTTCGCAATTTGTGAGGG	55.0	50.0	58.1	57.9	422-441 bp	1.0	84.6
atpI-rps2_p3	TCCACACCGGATACTTCGTA	GTGTTCGCAATTTGTGAGGG	50.0	50.0	57.9	57.9	420-439 bp	1.0	84.3
atpI-rps2_p4	ATGTTGGCCTACTTCCACAC	GTGTTCGCAATTTGTGAGGG	50.0	50.0	57.8	57.9	433-452 bp	1.0	83.6
atpI-rps2_p5	GTACTTGTCCGTGGACTTGG	GTGTTCGCAATTTGTGAGGG	55.0	50.0	58.2	57.9	476-495 bp	1.0	83.4