Hotspot

psbK-psbI

LSC · 7320-8043 · 724 bp

Back to Lasianthus

Pi 0.00856

MarkerSeek score 54.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 9
pi: 0.00856
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	TAAAGCGGCGGATTGCTAAT	45.0	45.0	58.1	58.0	1015-1103 bp	1.0	83.2
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.9	58.0	1010-1098 bp	1.0	83.2
psbK-psbI_p3	TTCTCTGGGACGGAAGGATT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.0	58.0	1594-1682 bp	1.0	82.0
psbK-psbI_p4	GCCGGTCATACCCGTTTTAT	TAAAGCGGCGGATTGCTAAT	50.0	45.0	58.0	58.0	1047-1088 bp	0.666667	69.9
psbK-psbI_p5	GCCGGTCATACCCGTTTTAT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.0	58.1	1071-1112 bp	0.666667	69.3