Hotspot

matK-trnK-UUU

LSC · 3253-3975 · 723 bp

Back to Lasianthus

Pi 0.00369

MarkerSeek score 61.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 4
pi: 0.00369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACCGGGTTGTTGCGATATAC	TTGGTCTTCGGGTTGCTAAC	50.0	50.0	58.1	58.1	820 bp	1.0	79.0
matK-trnK-UUU_p2	CGGGTTGTTGCGATATACCA	TTGGTCTTCGGGTTGCTAAC	50.0	50.0	58.1	58.1	818 bp	1.0	79.0
matK-trnK-UUU_p3	ACCGGGTTGTTGCGATATAC	AACGGTAGAGTACTCGGCTT	50.0	50.0	58.1	58.2	798 bp	1.0	78.6
matK-trnK-UUU_p4	ACCGGGTTGTTGCGATATAC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.1	58.2	797 bp	1.0	78.6
matK-trnK-UUU_p5	CGGGTTGTTGCGATATACCA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	58.1	58.2	795 bp	1.0	78.6