Hotspot

rpl16

LSC · 81613-82984 · 1372 bp

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Pi 0.00219

MarkerSeek score 45.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1372
variable_sites: 3
pi: 0.00219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CTGACCACCTCTGATCTTGC	GGGTTCCCCTTCAAACCATT	55.0	50.0	58.0	58.0	1922-1937 bp	1.0	80.7
rpl16_p2	ACACATCAATTCTCGAGCCC	GGGTTCCCCTTCAAACCATT	50.0	50.0	58.0	58.0	1809-1824 bp	1.0	80.7
rpl16_p3	TCGACGATTGCTAGCTCCTA	GGGTTCCCCTTCAAACCATT	50.0	50.0	58.0	58.0	1839-1854 bp	1.0	80.6
rpl16_p4	CTGACCACCTCTGATCTTGC	GTCGAGGGAATTGCACGTAT	55.0	50.0	58.0	58.1	2301-2316 bp	1.0	80.2
rpl16_p5	ACACATCAATTCTCGAGCCC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2188-2203 bp	1.0	80.2