Hotspot

petD

LSC · 76908-78154 · 1247 bp

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Pi 0.0016
MarkerSeek score 59.70
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1247
variable_sites
2
pi
0.0016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 ATTTCCCTTGAACACTGCAA TCCCTAGATACACAGGTCGT 40.0 50.0 55.7 56.9 1373 bp 1.0 53.1
petD_p2 TTTCCCTTGAACACTGCAAT TCCCTAGATACACAGGTCGT 40.0 50.0 55.7 56.9 1372 bp 1.0 53.1
petD_p3 ATTTCCCTTGAACACTGCAA GGGACAATTCGCTTTGTTTGA 40.0 42.9 55.7 57.6 1403 bp 1.0 51.1
petD_p4 TTTCCCTTGAACACTGCAAT GGGACAATTCGCTTTGTTTGA 40.0 42.9 55.7 57.6 1402 bp 1.0 51.1
petD_p5 TTTCCCTTGAACACTGCAAT AGGGACAATTCGCTTTGTTTG 40.0 42.9 55.7 57.6 1403 bp 1.0 51.1