Hotspot

atpB-rbcL

LSC · 55086-55843 · 758 bp

Back to Laserpitium

Pi 0.00264

MarkerSeek score 60.40

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 2
pi: 0.00264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCGGTGGAAAAGCTACATCT	GCTTTGAATCCAACACCTGC	50.0	50.0	57.6	57.9	914-921 bp	1.0	76.5
atpB-rbcL_p2	CCCGGTGGAAAAGCTACATC	GCTTTGAATCCAACACCTGC	55.0	50.0	58.6	57.9	915-922 bp	1.0	74.7
atpB-rbcL_p3	CCGGTGGAAAAGCTACATCT	ACCCCAGCTTTGAATCCAAC	50.0	50.0	57.6	58.4	920-927 bp	1.0	74.1
atpB-rbcL_p4	CCGGTGGAAAAGCTACATCT	CCCCAGCTTTGAATCCAACA	50.0	50.0	57.6	58.4	919-926 bp	1.0	74.1
atpB-rbcL_p5	CCGGTGGAAAAGCTACATCT	AACCCCAGCTTTGAATCCAA	50.0	45.0	57.6	57.6	921-928 bp	1.0	73.9