Hotspot

trnT-GGU-psbD

LSC · 32155-33408 · 1254 bp

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Pi 0.00159
MarkerSeek score 59.70
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1254
variable_sites
2
pi
0.00159
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AAGGGCCCTTTTAACTCAGC AGACCGGACCATCCTACAAA 50.0 50.0 58.1 58.1 1447-1452 bp 1.0 79.1
trnT-GGU-psbD_p2 GAATTGAAGCAAAAGGGCCC AGACCGGACCATCCTACAAA 50.0 50.0 57.9 58.1 1459-1464 bp 1.0 78.9
trnT-GGU-psbD_p3 GGAATTGAAGCAAAAGGGCC AGACCGGACCATCCTACAAA 50.0 50.0 57.9 58.1 1460-1465 bp 1.0 78.9
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA AGACCGGACCATCCTACAAA 50.0 50.0 57.9 58.1 1443-1448 bp 1.0 78.9
trnT-GGU-psbD_p5 AAGGGCCCTTTTAACTCAGC GAGCAATAGACCGGACCATC 50.0 55.0 58.1 57.8 1454-1459 bp 1.0 78.3