Hotspot

ycf1

Genome · 65221-71880 · 6660 bp

Pi 0.00622

MarkerSeek score 60.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 6660
variable_sites: 137
pi: 0.00622
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGGCAATGAACAGGTCCTA	GTCTCATCAATCCCCTGCTG	50.0	55.0	58.3	58.0	1172-1241 bp	1.0	84.8
ycf1_p2	AGGGCAATGAACAGGTCCTA	GGTACCCTTCCAATCCTCCT	50.0	55.0	58.3	58.1	861-942 bp	1.0	84.7
ycf1_p3	AGGGCAATGAACAGGTCCTA	ATCCGGATCCTCTCTTCTCG	50.0	55.0	58.3	58.1	1231-1300 bp	1.0	84.2
ycf1_p4	AGGGCAATGAACAGGTCCTA	CCGGATCCTCTCTTCTCGAT	50.0	55.0	58.3	58.1	1229-1298 bp	1.0	84.2
ycf1_p5	AGGGCAATGAACAGGTCCTA	TGATTCCCAAAAGTTCCGGG	50.0	50.0	58.3	58.1	1273-1342 bp	1.0	84.1