Hotspot

ycf4-cemA

Genome · 111323-112174 · 852 bp

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Pi 0.00391
MarkerSeek score 51.60
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
Genome
length_bp
852
variable_sites
14
pi
0.00391
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 TGCGTGTATCCATTGAAGGG TTTGGCCACTTCCAATTCGT 50.0 45.0 58.0 58.3 983-987 bp 1.0 78.5
ycf4-cemA_p2 GCGTGTATCCATTGAAGGGT TTTGGCCACTTCCAATTCGT 50.0 45.0 58.0 58.3 982-986 bp 1.0 78.5
ycf4-cemA_p3 TGCGTGTATCCATTGAAGGG CGAACCTGATTCACTCGTCA 50.0 50.0 58.0 57.7 953-957 bp 1.0 78.2
ycf4-cemA_p4 GCGTGTATCCATTGAAGGGT CGAACCTGATTCACTCGTCA 50.0 50.0 58.0 57.7 952-956 bp 1.0 78.2
ycf4-cemA_p5 TGCGTGTATCCATTGAAGGG ACGAACCTGATTCACTCGTC 50.0 50.0 58.0 57.7 954-958 bp 0.916667 74.7