Hotspot

atpB-rbcL

Genome · 105840-106594 · 755 bp

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Pi 0.00195
MarkerSeek score 53.70
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
Genome
length_bp
755
variable_sites
7
pi
0.00195
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CGGAAACCCCAAGAACAAGA CCAGCTTTGAATCCGACACT 50.0 50.0 58.0 58.2 777-861 bp 1.0 83.0
atpB-rbcL_p2 ATTTGAGCGATACGTCCCAC CCAGCTTTGAATCCGACACT 50.0 50.0 58.1 58.2 814-898 bp 1.0 82.7
atpB-rbcL_p3 CGGAAACCCCAAGAACAAGA AACACCAGCTTTGAATCCGA 50.0 45.0 58.0 57.7 781-865 bp 1.0 82.3
atpB-rbcL_p4 ATTTGAGCGATACGTCCCAC AACACCAGCTTTGAATCCGA 50.0 45.0 58.1 57.7 818-902 bp 1.0 82.0
atpB-rbcL_p5 GATTTGAGCGATACGTCCCA CCAGCTTTGAATCCGACACT 50.0 50.0 57.8 58.2 815-899 bp 1.0 81.4