Hotspot

trnL-CAA-ycf2

Genome · 84150-84763 · 614 bp

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Pi 0.00358
MarkerSeek score 51.80
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
Genome
length_bp
614
variable_sites
8
pi
0.00358
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC ATCAGCCGGGAGAATCTGTA 50.0 50.0 58.0 57.9 698-709 bp 1.0 81.7
trnL-CAA-ycf2_p2 TGAAATGGTAGACACGCGAG ATCAGCCGGGAGAATCTGTA 50.0 50.0 58.0 57.9 700-711 bp 1.0 81.7
trnL-CAA-ycf2_p3 AGGATTCACTTTCGGGATGC ATCAGCCGGGAGAATCTGTA 50.0 50.0 58.0 57.9 728-739 bp 1.0 81.3
trnL-CAA-ycf2_p4 GATTCACTTTCGGGATGCCT ATCAGCCGGGAGAATCTGTA 50.0 50.0 58.0 57.9 726-737 bp 1.0 81.3
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC TCAGCCGGGAGAATCTGTAA 50.0 50.0 58.0 58.1 697-708 bp 1.0 81.3