Hotspot

ndhB-trnL-CAA

Genome · 82473-84068 · 1596 bp

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Pi 0.00277
MarkerSeek score 57.70
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
Genome
length_bp
1596
variable_sites
17
pi
0.00277
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 GAAGTTATCTTTCCCCGGCC GTCTCGCGTGTCTACCATTT 55.0 50.0 58.3 58.0 1652-1662 bp 1.0 78.9
ndhB-trnL-CAA_p2 GGAAGTTATCTTTCCCCGGC GTCTCGCGTGTCTACCATTT 55.0 50.0 58.3 58.0 1653-1663 bp 1.0 78.9
ndhB-trnL-CAA_p3 GAAGTTATCTTTCCCCGGCC CTCGCGTGTCTACCATTTCA 55.0 50.0 58.3 58.0 1650-1660 bp 1.0 78.9
ndhB-trnL-CAA_p4 GGAAGTTATCTTTCCCCGGC CTCGCGTGTCTACCATTTCA 55.0 50.0 58.3 58.0 1651-1661 bp 1.0 78.9
ndhB-trnL-CAA_p5 TATCTTTCCCCGGCCAGAAT GTCTCGCGTGTCTACCATTT 50.0 50.0 58.5 58.0 1647-1657 bp 1.0 77.7