Hotspot

trnV-GAC-rps12

Genome · 79129-80718 · 1590 bp

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Pi 0.00287
MarkerSeek score 59.80
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
Genome
length_bp
1590
variable_sites
16
pi
0.00287
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1673-1688 bp 1.0 78.5
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1677-1692 bp 1.0 78.5
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1672-1687 bp 1.0 78.5
trnV-GAC-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1675-1690 bp 1.0 77.6
trnV-GAC-rps12_p5 GGCTCGAACTGATGACTTCC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.4 57.7 1683-1698 bp 1.0 77.0