Hotspot

trnP-GGG-rpl32

Genome · 76600-77706 · 1107 bp

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Pi 0.0021
MarkerSeek score 56.80
Primer available yes
Label trnP-GGG-rpl32

Feature Metadata

label_name
trnP-GGG-rpl32
feature_type
igs
region
Genome
length_bp
1107
variable_sites
10
pi
0.0021
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-rpl32_p1 TGGTTATAACGGAGCATGGC AAGTCTATCTCCACCGGTCA 50.0 50.0 58.0 57.5 767-1298 bp 1.0 80.1
trnP-GGG-rpl32_p2 TGGTTATAACGGAGCATGGC AGTCTATCTCCACCGGTCAT 50.0 50.0 58.0 57.2 766-1297 bp 1.0 78.3
trnP-GGG-rpl32_p3 TGGTTATAACGGAGCATGGC TCTCCACCGGTCATTCAAAA 50.0 45.0 58.0 57.1 760-1291 bp 1.0 76.8
trnP-GGG-rpl32_p4 TGGTTATAACGGAGCATGGC TCTATCTCCACCGGTCATTCA 50.0 47.6 58.0 58.0 764-1295 bp 1.0 76.1
trnP-GGG-rpl32_p5 TGGTTATAACGGAGCATGGC AGTCTATCTCCACCGGTCATT 50.0 47.6 58.0 58.0 766-1297 bp 1.0 76.1