Hotspot

ndhD

SSC · 109379-110935 · 1557 bp

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Pi 0.0024

MarkerSeek score 29.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1557
variable_sites: 19
pi: 0.0024
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGTGGCGTCAATAGGCTTT	TGATACCTTGGGACGGATGT	45.0	50.0	58.1	58.1	2030-2058 bp	1.0	80.6
ndhD_p2	ATCCGCCGTTTTTCTTCAGT	TGATACCTTGGGACGGATGT	45.0	50.0	58.1	58.1	2357-2376 bp	1.0	80.6
ndhD_p3	AATTGTGGCGTCAATAGGCT	TGATACCTTGGGACGGATGT	45.0	50.0	57.9	58.1	2032-2060 bp	1.0	80.3
ndhD_p4	ATTGTGGCGTCAATAGGCTT	TGATACCTTGGGACGGATGT	45.0	50.0	57.9	58.1	2031-2059 bp	1.0	80.3
ndhD_p5	CAATTGTGGCGTCAATAGGC	TGATACCTTGGGACGGATGT	50.0	50.0	57.8	58.1	2033-2061 bp	1.0	79.8