Hotspot

ccsA-ndhD

SSC · 109235-109378 · 144 bp

Back to Lappula

Pi 0.0458

MarkerSeek score 55.90

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 144
variable_sites: 22
pi: 0.0458
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTGTGGCGTCAATAGGCTTT	GAGCATATGGGTTGGTTCGT	45.0	50.0	58.1	58.0	990-1008 bp	1.0	82.2
ccsA-ndhD_p2	ATCCGCCGTTTTTCTTCAGT	GAGCATATGGGTTGGTTCGT	45.0	50.0	58.1	58.0	1317-1332 bp	1.0	82.0
ccsA-ndhD_p3	AATTGTGGCGTCAATAGGCT	GAGCATATGGGTTGGTTCGT	45.0	50.0	57.9	58.0	992-1010 bp	1.0	82.0
ccsA-ndhD_p4	ATTGTGGCGTCAATAGGCTT	GAGCATATGGGTTGGTTCGT	45.0	50.0	57.9	58.0	991-1009 bp	1.0	82.0
ccsA-ndhD_p5	TTGTGGCGTCAATAGGCTTT	TAACATCTCTTGGCCAACGG	45.0	50.0	58.1	57.9	882-900 bp	0.973684	80.8