Hotspot

ycf4-cemA

LSC · 56813-57511 · 699 bp

Back to Lappula

Pi 0.00714

MarkerSeek score 55.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 24
pi: 0.00714
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	TGAGAGAGAAACCCACCAGG	55.0	55.0	58.0	58.6	829-1065 bp	1.0	80.6
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	TGAGAGAGAAACCCACCAGG	50.0	55.0	58.1	58.6	828-1064 bp	1.0	80.4
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	TGAGAGAGAAACCCACCAGG	50.0	55.0	58.1	58.6	827-1063 bp	1.0	80.4
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	TGAGAGAGAAACCCACCAGG	45.0	55.0	58.1	58.6	765-1001 bp	1.0	80.2
ycf4-cemA_p5	ACAAAAAGCTGCCGAATTGG	TGAGAGAGAAACCCACCAGG	45.0	55.0	57.8	58.6	781-1017 bp	1.0	79.7