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trnK-UUU-rps16

LSC · 4102-4846 · 745 bp

Back to Lappula

Pi 0.00483

MarkerSeek score 55.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 24
pi: 0.00483
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCGATGGTATGGACGAATCC	GGTGCTCAACCTACAGGAAC	55.0	55.0	57.9	58.2	903-966 bp	1.0	83.9
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	842-905 bp	1.0	83.3
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	841-904 bp	1.0	83.3
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	851-914 bp	1.0	82.4
trnK-UUU-rps16_p5	TGGACGAATCCTTCCCTTCA	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.3	58.2	894-957 bp	1.0	81.7