Hotspot

ycf1

IRa · 123924-129506 · 5583 bp

Back to Laportea

Pi 0.109

MarkerSeek score 77.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 5583
variable_sites: 1557
pi: 0.109
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCACAAGTTATGCACTTCCT	GGGGAATGGAAACCGAACTT	45.0	50.0	56.3	58.1	301-361 bp	0.3	45.3
ycf1_p2	GCACAAGTTATGCACTTCCT	AACCGTTTGGGGAATGGAAA	45.0	45.0	56.3	57.9	309-369 bp	0.3	45.2
ycf1_p3	GCACAAGTTATGCACTTCCT	AATAGCCGAAGCCTGGGATA	45.0	50.0	56.3	58.3	672-744 bp	0.3	42.6
ycf1_p4	GCACAAGTTATGCACTTCCT	TTTGGAACGAACCGAACCTT	45.0	45.0	56.3	58.0	930-936 bp	0.2	35.1
ycf1_p5	GCACAAGTTATGCACTTCCT	TTTTGGAACGAACCGAACCT	45.0	45.0	56.3	58.0	931-937 bp	0.2	35.1