Hotspot

rpl16

LSC · 79977-81270 · 1294 bp

Back to Laportea

Pi 0.0641
MarkerSeek score 83.70
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1294
variable_sites
230
pi
0.0641
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TTTCTGGCTCCGCTGTTATC ATGGATCCGGGAAGGTAGAG 50.0 55.0 58.0 58.0 1720-1891 bp 0.8 83.7
rpl16_p2 AGCGTCGATTACTAGCTCCT ATGGATCCGGGAAGGTAGAG 50.0 55.0 58.0 58.0 1761-1932 bp 0.8 83.6
rpl16_p3 TGTTTCAATGCAAAGGCGAC ATGGATCCGGGAAGGTAGAG 45.0 55.0 57.9 58.0 1652-1807 bp 0.6 74.8
rpl16_p4 TTTCTGGCTCCGCTGTTATC GCCGAATTTATAGCCGGACA 50.0 50.0 58.0 58.1 1869-2024 bp 0.4 63.8
rpl16_p5 TTTCTGGCTCCGCTGTTATC TGCCGAATTTATAGCCGGAC 50.0 50.0 58.0 58.1 1870-2025 bp 0.4 63.8