Hotspot

ndhF-rpl32

SSC · 112150-113064 · 915 bp

Back to Laportea

Pi 0.117

MarkerSeek score 76.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 915
variable_sites: 250
pi: 0.117
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTCCAACGCGATTAGTCACA	AATGCTGCCCAATAGCCTTT	50.0	45.0	58.0	58.1	1415-1649 bp	0.7	78.9
ndhF-rpl32_p2	CTCCAACGCGATTAGTCACA	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.0	57.8	1425-1659 bp	0.7	78.5
ndhF-rpl32_p3	CTCCAACGCGATTAGTCACA	ATGCTGCCCAATAGCCTTTT	50.0	45.0	58.0	58.1	1490-1648 bp	0.3	62.1
ndhF-rpl32_p4	AGATCCTTTGTTGCCTTGGG	AATGCTGCCCAATAGCCTTT	50.0	45.0	58.1	58.1	1108-1200 bp	0.2	54.8
ndhF-rpl32_p5	AGATCCTTTGTTGCCTTGGG	ATGCTGCCCAATAGCCTTTT	50.0	45.0	58.1	58.1	1107-1199 bp	0.2	54.8