Hotspot

psbE-petL

LSC · 63522-64601 · 1080 bp

Back to Laportea

Pi 0.106

MarkerSeek score 84.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1080
variable_sites: 300
pi: 0.106
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TCTCCTGTGCTTCCAGACAT	AGTAGAAAACCGAAATAACTAGT	50.0	30.4	58.4	53.2	909-1208 bp	1.0	52.0
psbE-petL_p2	TCTCCTGTGCTTCCAGACAT	ACCGAAATAACTAGTTATAGTAAGCA	50.0	30.8	58.4	56.1	901-1200 bp	1.0	52.0
psbE-petL_p3	TCTCCTGTGCTTCCAGACAT	AGTAGAAAACCGAAATAACTAGTT	50.0	29.2	58.4	54.0	909-1208 bp	1.0	52.0
psbE-petL_p4	TATCGGCAAAAGAACGCTCT	AGTAGAAAACCGAAATAACTAGT	45.0	30.4	57.6	53.2	1152 bp	0.1	4.0
psbE-petL_p5	TATCGGCAAAAGAACGCTCT	ACCGAAATAACTAGTTATAGTAAGCA	45.0	30.8	57.6	56.1	1144 bp	0.1	4.0