Hotspot

rbcL-accD

LSC · 55381-56105 · 725 bp

Back to Laportea

Pi 0.0843

MarkerSeek score 84.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 166
pi: 0.0843
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATACGAGTCTGGCTCA	TTGAACCCACAAATGCCTGT	50.0	45.0	58.0	58.2	1955-2037 bp	0.9	86.2
rbcL-accD_p2	TGCAAATACGAGTCTGGCTC	TTGAACCCACAAATGCCTGT	50.0	45.0	58.0	58.2	1956-2038 bp	0.9	86.2
rbcL-accD_p3	CTGCAAATACGAGTCTGGCT	TTGAACCCACAAATGCCTGT	50.0	45.0	58.0	58.2	1957-2039 bp	0.9	86.2
rbcL-accD_p4	GCCGTAGCTAATCGAGTAGC	TTGAACCCACAAATGCCTGT	55.0	45.0	58.0	58.2	1532-1614 bp	0.9	86.0
rbcL-accD_p5	ATATGCCTGCTTTGACCGAG	TTGAACCCACAAATGCCTGT	50.0	45.0	58.0	58.2	1624-1706 bp	0.9	86.0