Hotspot

atpB-rbcL

LSC · 53177-53931 · 755 bp

Back to Laportea

Pi 0.0773

MarkerSeek score 84.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 166
pi: 0.0773
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TCCATGAGCTTTGGCAATGT	CGGTCTCTCCAACGCATAAA	45.0	50.0	58.1	58.0	1996-2024 bp	1.0	91.4
atpB-rbcL_p2	ATTGTATAAAGGCAGGCGCA	CGGTCTCTCCAACGCATAAA	45.0	50.0	57.9	58.0	1838-1866 bp	0.9	87.6
atpB-rbcL_p3	ATTGTATAAAGGCAGGCGCA	CCCGTCAGTCCATACAGTTG	45.0	55.0	57.9	58.0	1407-1435 bp	0.9	87.5
atpB-rbcL_p4	TCCATGAGCTTTGGCAATGT	CCCGTCAGTCCATACAGTTG	45.0	55.0	58.1	58.0	1565-1593 bp	0.9	87.3
atpB-rbcL_p5	ATTGTATAAAGGCAGGCGCA	CAGGCTCAATGTGGTAGCAT	45.0	50.0	57.9	58.0	1456-1484 bp	0.9	87.2